2008Dadou kexueRequires access

Screening of Bacillus sp. Against Soybean Cyst Nematode(Heterodera glycines)

Shengjun Wang

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Abstract

The Soybean Cyst Nematode(Heterodera glycines),causes economically significant damage to soybean in China.Some bacteria Bacillus,are both resistant to advese envirnment and against pathogens.295 Bacillus sp.were isolated from soil samples by diluted separation in Northeast and North China.B12,B28,B49,B65,B76 and B108 strains could suppress Heterodera glycines and strain suspentions of B12 was effective significantly.The corrected mortality rate of nematodes of strain suspentions with B12 were 74.03% after 48 h.The corrected mortality rate of nematodes of fermentation filtrate with B65 were 85.90% after 48 h.The suppressive percentages of cysts hatching of B12,B65 and B49 were 89.50,89.50 and 88.00.The bacterial strains which had effect on the nematode would be used as the bio-control agents on the soybean root rot pathogen and soybean cyst nematode.

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The Soybean Cyst Nematode(Heterodera glycines),causes economically significant damage to soybean in China.Some bacteria Bacillus,are both resistant to advese envirnment and against pathogens.295 Bacillus sp.were isolated from soil samples by diluted separation in Northeast and North China.B12,B28,B49,B65,B76 and B108 strains could suppress Heterodera glycines and strain suspentions of B12 was effective significantly.The corrected mortality rate of nematodes of strain suspentions with B12 were 74.03% after 48 h.The corrected mortality rate of nematodes of fermentation filtrate with B65 were 85.90% after 48 h.The suppressive percentages of cysts hatching of B12,B65 and B49 were 89.50,89.50 and 88.00.The bacterial strains which had effect on the nematode would be used as the bio-control agents on the soybean root rot pathogen and soybean cyst nematode.

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Available abstract

The Soybean Cyst Nematode(Heterodera glycines),causes economically significant damage to soybean in China.Some bacteria Bacillus,are both resistant to advese envirnment and against pathogens.295 Bacillus sp.were isolated from soil samples by diluted separation in Northeast and North China.B12,B28,B49,B65,B76 and B108 strains could suppress Heterodera glycines and strain suspentions of B12 was effective significantly.The corrected mortality rate of nematodes of strain suspentions with B12 were 74.03% after 48 h.The corrected mortality rate of nematodes of fermentation filtrate with B65 were 85.90% after 48 h.The suppressive percentages of cysts hatching of B12,B65 and B49 were 89.50,89.50 and 88.00.The bacterial strains which had effect on the nematode would be used as the bio-control agents on the soybean root rot pathogen and soybean cyst nematode.

Key concepts: Heterodera, Nematode, Soybean cyst nematode, Biology, Biological pest control, Bacillus amyloliquefaciens, Hatching, Cyst

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