UV/NTG Mutagenesis for Breeding of A High Trehalose-producing Strain of Saccharomyces cerevisiae
Zhang Chun-ling
Abstract
Zhang Chun-ling
Abstract
A high trehalose-producing strain of Saccharomyces cerevisiae was bred via combined nitrosoguanidine (NTG)/UV mutagenesis. This strain had good genetic stability and a trehalose content of 19.8% (m/m dry yeast), which revealed the enhancement of 39.43%, 10.0% and 6.45% compared with the original strain, the strain screened by alone UV mutagenesis, and the strain screened by alone NTG mutagenesis, respectively.
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A high trehalose-producing strain of Saccharomyces cerevisiae was bred via combined nitrosoguanidine (NTG)/UV mutagenesis. This strain had good genetic stability and a trehalose content of 19.8% (m/m dry yeast), which revealed the enhancement of 39.43%, 10.0% and 6.45% compared with the original strain, the strain screened by alone UV mutagenesis, and the strain screened by alone NTG mutagenesis, respectively.
Key concepts: Mutagenesis, Trehalose, Strain (injury), Saccharomyces cerevisiae, Yeast, Biology, Mutation, Chemistry