The experimental study of cryopreserved Human umbilical cord mesenchymal stem cells on new born rats with hypoxic-ischemic brain encephalopathy

Objiective To observe the therapeutic effect of rats with hypoxic-ischemic brain damage(HIBD) by transplantation cryopreserved human umbilical cord mesenchymal stem cells(hUC-MSCs).Methods Ninety six 7-day-old neonate rats were randomly divided into 4 groups,normal control group(n=24),HIBD group(n=24),fresh hUC-MSCs transplantation group(n=24),cryopreserved hUC-MSCs transplantation group(n=24).The rats(except the normal control group) were established HIBD modles.Transplantation were carried out three days after HIE.Before transplantation,cells were determined by BrdU-incorporation.After transplantation,tissue incision and immunofluorescence were appeared to detect the survival of the transplanted cells in nivo,and sensorimotor function were measured to estimate the recovery of function.Results Cryopreserved hUC-MSCs had higher reproduction ability compared to fresh hUC-MSCs;tissue incision:brain damage was lighten in the transplantationg group compared to the HIBD group.Immunohistochemistry showed hUC-MSCs survived and migrated into the hypoxic-ischemic brain tissue,and can survived in the brain tissue at least 4 weeks;Sensorimotor functiong test: the difference between transplantation groups was not significant(P0.05),while transplantationg groups exhibited significant improvement(P0.05),compared with HIBD group.Conclusion Cryopreserved hUC-MSCs keeps the ability of reproduction and differentiation.hUC-MSCs is a kind of valuable cell resource for the therapy of HIBD.