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Citrus flavonoids simultaneous HPLC analysis

LU Yan-hua

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Abstract

AIM: To develop a rapid HPLC method for quality control of traditional Chinese medicinal ingredients consisted of citrus flavonoids,naringin,hesperidin,neohesperidin,sinensetin and nobiletin. METHODS: Gradient elution with non-salt mobile phase(methanol and water only) HPLC method on a Kromasil column(100-5C18-250A,4.6 mm×250 mm,5 μm,C_(18) reverse phase) with peaks identification through DAD full UV wavelength scan.UV 284 nm and 332 nm profiles were observed. RESULTS: Satisfactory resolution,linearity,95%～102% of recovery and 1.88～2.93% of repeatability were obtained for those five citrus flavonoids.Content of 6 Citrus aurantium L.based TCM ingredients were analyzed and identified. CONCLUSION: Rapid HPLC test method on citrus flavonoids was developed and can be in LC-MS identification.

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What this paper is about

AIM: To develop a rapid HPLC method for quality control of traditional Chinese medicinal ingredients consisted of citrus flavonoids,naringin,hesperidin,neohesperidin,sinensetin and nobiletin. METHODS: Gradient elution with non-salt mobile phase(methanol and water only) HPLC method on a Kromasil column(100-5C18-250A,4.6 mm×250 mm,5 μm,C_(18) reverse phase) with peaks identification through DAD full UV wavelength scan.UV 284 nm and 332 nm profiles were observed. RESULTS: Satisfactory resolution,linearity,95%～102% of recovery and 1.88～2.93% of repeatability were obtained for those five citrus flavonoids.Content of 6 Citrus aurantium L.based TCM ingredients were analyzed and identified. CONCLUSION: Rapid HPLC test method on citrus flavonoids was developed and can be in LC-MS identification.

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Available abstract

AIM: To develop a rapid HPLC method for quality control of traditional Chinese medicinal ingredients consisted of citrus flavonoids,naringin,hesperidin,neohesperidin,sinensetin and nobiletin. METHODS: Gradient elution with non-salt mobile phase(methanol and water only) HPLC method on a Kromasil column(100-5C18-250A,4.6 mm×250 mm,5 μm,C_(18) reverse phase) with peaks identification through DAD full UV wavelength scan.UV 284 nm and 332 nm profiles were observed. RESULTS: Satisfactory resolution,linearity,95%～102% of recovery and 1.88～2.93% of repeatability were obtained for those five citrus flavonoids.Content of 6 Citrus aurantium L.based TCM ingredients were analyzed and identified. CONCLUSION: Rapid HPLC test method on citrus flavonoids was developed and can be in LC-MS identification.

Key concepts: Hesperidin, Naringin, Nobiletin, Chromatography, Chemistry, High-performance liquid chromatography, Repeatability, Gradient elution

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