Studies on Reticular Framework of lymphonodus in the Mesenteric of Rat by Silver-impregnation

Objectives:To study the shape,density,arrangement and space contact of the reticular cells and the reticular fiber network in the lymphonodus.To research the tissue architecture and function of lymphonodus stroma.Methods:To observe stromal reticulum of lymphonodus in the mesenteric of rat with healthy and mature Wistar rat,using silver-impregnation methods by light microscope.Results:The stromal structural of lymphonodus is composed of reticular cells and fibers.The reticular fibers consist of thin bundles of fibrils,which were interwoven each other and linked with the fibrillar network of blood vessels,lymphatic sinuses,hitherto unknown lymphatic labyrinth as well as with the capsule of the lymphonodus,forming a three-dimensional reticular network throughout the organ and actor as a skeleton of the lymphonodus.There were regional differences between the reticular cells and the reticular fiber network in the shape,density,arrangement and space contact,including capsule and sub capsular sinus,superficial cortex,deep cortex and medulla,reticular network.They were linked each other.Conclusion:The arrangements of the reticular fibers in the rat mesenteric lymphonodus were closely related to the associated reticular cells.Variable reticular network forms the structural framework of the lymphonodus,which supports staying and moving of cells in the lymphonodus and provides a suitable microenvironment for cells staying.