Significance of Two ELISA Reagents in the Diagnosis of Hepatitis C Anti-HCV

Objective To raise the detection rate of the antibody of hepatitis C virus (anti-HCV) and the accuracy of test results.Methods HCV ELISA reagents produced by two manufacturers(reagents① and reagent ②) were used to test the 2 516 serum samples from patients before surgery.Then 28 serum samples (both reagents positive),15 samples (only reagent ① positive) and 17 samples(only reagent ② positive) were tested on HCV RNA by RT-PCR quantitative fluorescence.Results 28 samples which were positive to both reagents were RT-PCR positive,while only 2 of the 15 samples which were positive to only reagent ① were RT-PCR positive and 3 of the 17 samples which were positive to only reagent ② were RT-PCR positive.In the latter 32 samples,HCV RNA RT-PCR positive rate was 15.63%.Conclusions Double-reagents detection can enhance the anti-HCV detection rate and the accuracy of the results.