Clinical Application of Combined Detection of AnuA,ANA,anti ds-DNA and antiENA in SLE Patients

Objective To combine detection of SLE patients with a variety of autoantibodies,provide a basis for clinical diagnose and treatment. Methods AnuA,antiSm,antiSSA,antiSSB and antiRib were determined by western blotting,and antidsDNA and ANA were determined by indirect immunofluorescence(IIF) in 120 cases of SLE patients and 60 controls. Results The positive rates of ANA,anti dsDNA,AunA,antiSm,antiSSA,antiSSB and antiRib was 69.2%,70.8%,74.2%,35%,42.5%,11.7% and 26.7% in SLE patients. The positive rates of ANA,anti dsDNA and AunA was 90.7%,98.1% and 83.3% in patients with active stage of SLE. The positive rates of ANA, anti dsDNA and AunA was 51.5%,48.5% and 66.7% in patients with inactive stage of SLE. Three indicators had statistically significance in active stage and inactive stage(P0.01,P0.01 and P0.05). All of the test results of 60 controls were negative. Conclusion Combined detection of several autoantibody was important to diagnosis and curative evaluation in SLE.