Determination of Chlorantraniliprole Residue in Environmental Aquatic Samples by High Performance Liquid Chromatography

Chlorantraniliprole residues in the environmental aquatic system was extracted by liquid-liquid partition from water samples into dichloromethane,and determined by high performance liquid chromatography.The analysis was performed on a Kromasil C 18 stainless steel column(250mm×4.6mm i.d.,5μm) with mixture of methanol-water(65∶35,V/V)as the mobile phase at a flow rate of 1.0mL/min,at the column temperature of 35℃ and photodiode array detector at 236nm.The calibration curve for chlorantraniliprole showed a good linearity in the concentration range of 0.10—10mg/L with the regression equation y=2170.4x+2641.7,r2=0.9991.The average recovery of five spiked concentration levels for chlorantraniliprole in well water and pond water were 94.70%? ?00.37% and 89.14%—100.22%,respectively.The coefficient of variation(CV) was 0.81%? ?.11% and 1.81%—5.30%,respectively.The limit of quantitation(LOQ) for chlorantraniliprole was 7.5×10 -3 mg/L.The method was fast,sensitive,simple,reproducible and practical for the residue detection and analysis of chlorantraniliprole in aquatic systems.