2010•Zhongguo shiyan fangjixue zazhiRequires access

Studies on the Quality standard of Bimintong Capsule

Yan Ma

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Abstract

Objective: To establish the quality standard of Bimintong Capsule. Method: Rhizoma chuanxiong,Radix Angelicae pubescentis,and Radix saposhnikoviae were identified by TLC. The HPLC method was established to determine the content of osthole. A Shim-Pack ODS column was used with acetonitrile-water(60 ∶ 40) as mobile phases. The flow rate was 1. 0 mL·min -1. The detection wavelength was at 322 nm. Result: Rhizoma chuanxiong,Radix Angelicae pubescentis,and Radix saposhnikoviae could be identified by TLC and there were noninterference of the negative controls. Osthole was linear in the range of 5. 68 ~ 56. 80 μg·mL -1 ( r = 0. 999 8). The average recovery was 99. 27% ,and RSD 1. 01% (n = 6). Conclusion:The method established can be used as quality control for Bushen Jianpi Granule.

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Objective: To establish the quality standard of Bimintong Capsule. Method: Rhizoma chuanxiong,Radix Angelicae pubescentis,and Radix saposhnikoviae were identified by TLC. The HPLC method was established to determine the content of osthole. A Shim-Pack ODS column was used with acetonitrile-water(60 ∶ 40) as mobile phases. The flow rate was 1. 0 mL·min -1. The detection wavelength was at 322 nm. Result: Rhizoma chuanxiong,Radix Angelicae pubescentis,and Radix saposhnikoviae could be identified by TLC and there were noninterference of the negative controls. Osthole was linear in the range of 5. 68 ~ 56. 80 μg·mL -1 ( r = 0. 999 8). The average recovery was 99. 27% ,and RSD 1. 01% (n = 6). Conclusion:The method established can be used as quality control for Bushen Jianpi Granule.

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Available abstract

Objective: To establish the quality standard of Bimintong Capsule. Method: Rhizoma chuanxiong,Radix Angelicae pubescentis,and Radix saposhnikoviae were identified by TLC. The HPLC method was established to determine the content of osthole. A Shim-Pack ODS column was used with acetonitrile-water(60 ∶ 40) as mobile phases. The flow rate was 1. 0 mL·min -1. The detection wavelength was at 322 nm. Result: Rhizoma chuanxiong,Radix Angelicae pubescentis,and Radix saposhnikoviae could be identified by TLC and there were noninterference of the negative controls. Osthole was linear in the range of 5. 68 ~ 56. 80 μg·mL -1 ( r = 0. 999 8). The average recovery was 99. 27% ,and RSD 1. 01% (n = 6). Conclusion:The method established can be used as quality control for Bushen Jianpi Granule.

Key concepts: Radix (gastropod), Chromatography, Quality standard, Capsule, Chemistry, High-performance liquid chromatography, Linear range, Granule (geology)

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