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Analysis of Genetic Relationships of Pear Germplasm Resources in Xinjiang Based on ISSR and RAPD

Shan Jiang-hua

Open publisher page 2 citations

Abstract

【Objective】The research aimed to discuss the feasibility of classification and systematical development of pear cultivars by ISSR and RAPD.【Method】The Inter-simple sequence repeat(ISSR) and Random Amplified Polymorphic DNA(RAPD) molecular markers were used to analyze the genetic relationship of 48 main pears.【Result】101 polymorphic bands among 113 reproducible DNA bands were amplified by 14 ISSR primers with an average of 7.2 polymorphic bands per primer,and 138 polymorphic bands among 163 reproducible DNA bands were amplified y19 RAPD primers with 7.2 bands on average.Using cluster analysis(UPGMA) based on those polymorphism bands amplified with ISSR and RAPD primers,the 48 accessions were classified into three groups,group Ⅰwith 1 accessions,group Ⅱwith 23 accessions,and group Ⅲwith 24 accessions.【Conclusion】The two molecular markers are suitable for analysis of genetic relationships and genetic diversity of pear.The study will provide a reference for utilization and new variety selection of pear resources.

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What this paper is about

【Objective】The research aimed to discuss the feasibility of classification and systematical development of pear cultivars by ISSR and RAPD.【Method】The Inter-simple sequence repeat(ISSR) and Random Amplified Polymorphic DNA(RAPD) molecular markers were used to analyze the genetic relationship of 48 main pears.【Result】101 polymorphic bands among 113 reproducible DNA bands were amplified by 14 ISSR primers with an average of 7.2 polymorphic bands per primer,and 138 polymorphic bands among 163 reproducible DNA bands were amplified y19 RAPD primers with 7.2 bands on average.Using cluster analysis(UPGMA) based on those polymorphism bands amplified with ISSR and RAPD primers,the 48 accessions were classified into three groups,group Ⅰwith 1 accessions,group Ⅱwith 23 accessions,and group Ⅲwith 24 accessions.【Conclusion】The two molecular markers are suitable for analysis of genetic relationships and genetic diversity of pear.The study will provide a reference for utilization and new variety selection of pear resources.

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Available abstract

【Objective】The research aimed to discuss the feasibility of classification and systematical development of pear cultivars by ISSR and RAPD.【Method】The Inter-simple sequence repeat(ISSR) and Random Amplified Polymorphic DNA(RAPD) molecular markers were used to analyze the genetic relationship of 48 main pears.【Result】101 polymorphic bands among 113 reproducible DNA bands were amplified by 14 ISSR primers with an average of 7.2 polymorphic bands per primer,and 138 polymorphic bands among 163 reproducible DNA bands were amplified y19 RAPD primers with 7.2 bands on average.Using cluster analysis(UPGMA) based on those polymorphism bands amplified with ISSR and RAPD primers,the 48 accessions were classified into three groups,group Ⅰwith 1 accessions,group Ⅱwith 23 accessions,and group Ⅲwith 24 accessions.【Conclusion】The two molecular markers are suitable for analysis of genetic relationships and genetic diversity of pear.The study will provide a reference for utilization and new variety selection of pear resources.

Key concepts: RAPD, UPGMA, PEAR, Genetic diversity, Biology, Germplasm, Primer (cosmetics), Genetic relationship

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