A method of clastic anatomy of gastric muscular layers in animals

An anatomic method was set up to study the distribution,variance of thickness and connective way of gastric tunica muscularis in rabbits,dogs and pigs.Stomachs were fixed in 10 % formalin by using the turgor fixation and the no-tension fixation.Then,tunica serosa,tunica mucosa and tunica submucosa were striped so that intact tunica muscularis were reserved.The gastric tunica muscularis were striped from superficies externa or interna,respectively.The outer longitudinal muscular layer was striped,so that the outer longitudinal muscular layer and the middle circular muscular layer were observed clearly.And the internal oblique muscular layer was striped to observe the internal oblique muscular layer and the middle circular muscular layer clearly.The macroscopic observation was combined with the stereoscopic observation to study the gastric tunica muscularis.The principle of dissecting was from the bigger thickness of muscular fibers to smaller,from higher density of muscular fibers to lower,and along the muscular fibers.The conclusion was that the distribution of the gastric muscular layers was disproportion,and the thickness of the muscular layers was various in the different regions,and the connections of muscular fibers were non-uniform between every two gastric muscular layers.