Induction of human ovarian carcinoma cell line SKVO-3 apoptosis by siRNA through targeting to survivin

Objective To screen small interference RNAs that could inhibit survivin expression and to explore the effect of RNA interference of survivin on apoptosis of ovarian carcinoma carcinoma.Methods Three siRNA target sequences for survivin were designed and the siRNA expression cassettes were prepared by PCR.The siRNA expression cassettes were transfected into 293T cells along with survivin and green fluorescent protein fusion gene expression plasmid.The expression vector of an effective siRNA was constructed and transfected into ovarian carcinoma SKVO-3 cells,and the expression of survivin and apoptosis were assayed.Results A candidate siRNA that down-regulated the expression of survivin in SKVO-3 cells was selected,which could inhibit expression of survivin in SKVO-3 cells and lead to apoptosis actively.Conclusion siRNA can suppress expression of survivin and induce SKVO-3 cells apoptosis effectively,which might lay a foundation for further research on the treatment of malignant tumor through targeting to survivin.