Phenotype of mouse CD4~(+)CD25~(+) regulatory T cells in mouse splenocytes

To compare the phenotype and cytokines expression in CD4~(+)CD25~(+)T and CD4~(+)CD25~(-)T cells,single cell suspensions from mouse spleen were prepared and the CD4~(+)、CD4~(+)CD25~(+)、CD4~(+)CD25~(-)T cells were isolated.The expression of surface molecules,transcriptional factors and cytokines was determined and analysed at the single cell level by FACS.It was found that 7.8% of CD4~(+)T cells expressed CD25.Compared with CD4~(+)CD25~(-)T cells,expression of CD44 on CD4~(+)CD25~(+)T cells increased slightly,CD45RB decreased remarkably,CTLA-4 and Foxp3 increased significantly.The majority of CD4~(+)CD25~(+)T cells expressed both CTLA-4 and Foxp3,and much less cells expressed Foxp3 alone.The results of intracellular cytokines staining indicated that the frequency of IL-2~(+) and IFN-γ~(+) CD4~(+)CD25~(+)T cells decreased remarkably,and the frequency of IL-10~(+) CD4~(+)CD25~(+)T cells increased lightly compared with CD4~(+)CD25~(-)T cells.It is evident that the CD4~(+)CD25~(+)regulatory T cells differs both in the phenotype and the expressions of transcriptional factors and cytokines,with the CD4~(+)CD25~(-) non-regulatory T cells cells.It was different in phenotype、expression of transcriptional factors and cytokines in CD4~(+)CD25~(+)T cells and CD4~(+)CD25~(-)T cells.