Studies on Pollination Fruiting Characteristics of Dendrobium officinale

[Objective] To study pollination fruiting characteristics and to provide the theoretical basis and applied technology for production and breeding of Dendrobium officinale.[Method] Different pollination methods such as cross,self and subsidiary pollination by insects etc were carried out under the condition of greenhouse protected cultivation and tree-attached ecological cultivation,fruit set percentage was used to test the pollen vigor when pollination performed by using inflorescences stored in 4 ℃ or taking 75% alcohol or clean water as adhesion adjuvant. [Result]The setting percentage of cross,self and opening pollination were 90. 51%,5. 55% and 1. 82%,respectively. 94. 22% was the average rate of cold storage pollen within 9 days,no obvious changes occurred in pollen vitality with the cold storage time. Using 75% alcohol or clean water as adjuvant didn't affect pollination effects,the average rate was 91. 29%.[Conclusion]Cross-pollination was the main pattern of D. officinale,existing partially self-compatible as well. The fructification rate were low both in self and opening pollination,it's an effective way to protect and breed germplasm resources of D. officinale through the artificial cross pollination.