Expression of HIV-1 gag gene in E.Coli

Objective To express HIV-1 gag protein in E.Coli.Methods The truncated gag gene was inserted into the expressing vector Pet-28a and E.coli B1.21(DE3) was then transformed and IPTG was induced.The expressing product by SDS-PAGE and Western-Blot was detected.Results The recombinant plasmid was expressed with about 50 kD fusion protein which could react specifically with the HIV positive serum.Couclusion The truncated HIV-1 gag gene can be expressed in E.coli and the recombinant protein has positive antigenicity.