The Protective Effect of Ginkgolide B on Intestinal Oxidative Injury in Young Rats with Endotoxemia

Objective Oxidative stress is closely correlated with intestinal injury induced by inflammatory response.We investigated the protective effect of platelet activating factor receptor antagonist(Ginkgolide B,BN52021) on oxidative injury of intestinal in young rat with endotoxaemia.Methods Eighteen-day-old Wistar rats were randomly divided into 3 groups and treated with lipopolysaccharide(LPS)(5 mg/kg),LPS plus PAF receptor antagonist and normal saline injection(Control) respectively.Ginkgolide B 5 mg/kg was administered before or 30 minutes after LPS injection(pretreatment or treatment).The ileum specimens(n =8) were harvested at 1.5,3,6,24,48 and 72 h after LPS injection.Hematoxylin and erosin staining and transmission electron microscopy(TEM) was used for histological morphology evaluation.The rest samples were stored in-80 ℃ for measurements of xanthine oxidase(XOD) and superoxide dismutase(SOD).Results Histologic examination of intestinal injury in LPS group showed edema of the villus,capillary congestion of the lamina propria,extension of the subepithelial,polymorphonuclear infiltration in enteric cavity on 1.5,3,6,24 h.Edemas of the villus were found in antagonist group.Ultramicrostructural change of TEM showed microvilli and tight junctions were intact in the control group.Enlargement of tight junctions were seen in the experiment group,and the microvilli were thin,rare or disrupt,shed.The rough endoplasmic reticulum,mitochondria,and glycogen particles were injured.The levels of XOD at 1.5,3,6,24 h were obviously higher in LPS group than control group.The content of XOD increased mostly at 6 h from(239.85±17.64)U/g to(330.99±61.73)U/g of control group(P 0.01).The tendency of antagonist group was the same as the LPS group.But the levels of XOD of antagonist group were lower than LPS group at each time point.The levels of SOD of 1.5,3,6,24 and 48 h were obviously decreased in LPS group compared with control group,statistic significances were found.The content of SOD decreased from(141.55±28.12)U/mg to(86.89±9.99)U/mg of control group at 6 h(P 0.01).The tendency of antagonist group was the same as that of LPS group.But the levels of SOD of antagonist group were higher than LPS group at each point.Conclusions Oxidative damage plays a role in intestinal injury during endotoxemia.Preventive and remedial application of PAF receptor antagonist(Ginkgolide B) may relieve intestinal inflammatory response,and have a protective effect on intestinal mucosal injury.