Serotype,virulence and drug resistance of Vibrio parahaemolyticus from food contaminant

Objective:To understand the serogroup distribution,toxin-producing characteristics and resistance to antibiotics of the Vibrio parahaemolyticus isolated from food contaminant,and provide scientific evidence for prevention and treatment of disease caused by V.parahaemolyticus.Methods: Using the standard technique GB/T 4789.7-2008,serotypes were determined by standard serum and virulence genes tdh,trh and tlh were also determined by multiple PCR.Agar diffusion method was used to test the antibiotic susceptibility.Results: 35 strains of Vibrio parahaemolyticus consisted of nine serum groups:O:4 28.6%(10/35)、O:1 20%(7/35)、O:3 14.3%(5/35),O:5 14.3%(5/35),O:2 8.6%(3/35),O:10 5.7%(2/35),O:11 2.9%(1/35),O:6 2.9%(1/35),O:8 2.9%(1/35).Tdh and trh is negative in any of the 35 strains,tlh all positive.Susceptibility tests showed that 35 VP strains were resistant to 20 kinds of antibiotics,with the highest resistance to ampicillin(97.1%).Conclusion: The VP isolated from food pollutant in Wenzhou City have diversified characteristics of serological type.Should be established to prevent any food of food-borne illnesses by V.parahaemolyticus.Proposal to strengthen the supervision and inspection food contaminant.