2011•Food ScienceRequires access
Mixed Design Optimization of Aqueous Enzymatic Extraction of Soybean Oil with Enzyme Complex
Wang Wen-rui
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Abstract
Cellulose,hemicellulase and were simultaneously used for the aqueous enzymatic extraction of soybean oil by in the present study,and the extraction process was optimized by mixed design.It was found that the optimal hydrolysis process for soybean oil extraction consisted of suspending of soybean powder in a 6-fold volume of water,adding 0.84% cellulose and 0.56% hemi-cellulase for hydrolysis at 37 ℃ for 0.75 h,and further hydrolysis with Alcalase added at a concentration of 1.85% for 3.6 h at 50 ℃with an initial pH of 9.26,and provided an oil extraction efficiency of 81.04%,which was higher than that of wet-heating treatment extraction.