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2001•Chinese journal of integrated traditional and Western medicineRequires access

Serapharmacological Study of Ganzheng Peroral Solution on Proliferation and Apoptosis of Human Hepatocellular Carcinoma SMMC-7721 Cells with Transforming Growth Factor α

Hongsheng Gao

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Abstract

Objective: To investigate the serapharmacological effects of Ganzheng Peroral Solution(GZPS) on proliferation and apoptosis of human hepatocellular carcinoma SMMC 7721 cells with transforming growth factor α(TGFα).Methods:The effects of GZPS were investigated in vitro using serum pharmacological approach compared with pathologic serum. The inhibiting effect of drug serum on SMMC 7721 cells with TGFα was observed by MTT assay. Cell apoptosis and cell cycling analysis were detected by flow cytometry.Results: The drug serum could significantly inhibit the proliferation of SMMC 7721 cells with TGFα time dependently. It also could increase the apoptosis rate and arrest cell cycle at G 0/1 phase so that it could decrease S phase fraction(SPF) and proliferous index(PI).Conclusion:The serum containing GZPS could inhibit the proliferation of SMMC 7721 cells with TGFα and increase the apoptosis rate.

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What this paper is about

Objective: To investigate the serapharmacological effects of Ganzheng Peroral Solution(GZPS) on proliferation and apoptosis of human hepatocellular carcinoma SMMC 7721 cells with transforming growth factor α(TGFα).Methods:The effects of GZPS were investigated in vitro using serum pharmacological approach compared with pathologic serum. The inhibiting effect of drug serum on SMMC 7721 cells with TGFα was observed by MTT assay. Cell apoptosis and cell cycling analysis were detected by flow cytometry.Results: The drug serum could significantly inhibit the proliferation of SMMC 7721 cells with TGFα time dependently. It also could increase the apoptosis rate and arrest cell cycle at G 0/1 phase so that it could decrease S phase fraction(SPF) and proliferous index(PI).Conclusion:The serum containing GZPS could inhibit the proliferation of SMMC 7721 cells with TGFα and increase the apoptosis rate.

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Available abstract

Objective: To investigate the serapharmacological effects of Ganzheng Peroral Solution(GZPS) on proliferation and apoptosis of human hepatocellular carcinoma SMMC 7721 cells with transforming growth factor α(TGFα).Methods:The effects of GZPS were investigated in vitro using serum pharmacological approach compared with pathologic serum. The inhibiting effect of drug serum on SMMC 7721 cells with TGFα was observed by MTT assay. Cell apoptosis and cell cycling analysis were detected by flow cytometry.Results: The drug serum could significantly inhibit the proliferation of SMMC 7721 cells with TGFα time dependently. It also could increase the apoptosis rate and arrest cell cycle at G 0/1 phase so that it could decrease S phase fraction(SPF) and proliferous index(PI).Conclusion:The serum containing GZPS could inhibit the proliferation of SMMC 7721 cells with TGFα and increase the apoptosis rate.

Key concepts: Apoptosis, Flow cytometry, Hepatocellular carcinoma, Cell cycle, Transforming growth factor, Cell growth, In vitro, MTT assay

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Serapharmacological Study of Ganzheng Peroral Solution on Proliferation and Apoptosis of Human Hepatocellular Carcinoma SMMC-7721 Cells with Transforming Growth Factor α — Research Paper | ScholarLens