The primary culture for human embryonic olfactory ensheathing cells of olfactory bulb

Objective To establish a cultural method for human embryonic olfactory ensheathing cells(OECs)of olfactory bulb in order to obtain cells with better viability and purification to satisfy the clinical application.Methods The olfactory ensheathing cells were purified with serum-free purification liquid containing glutamine,transferrin,biotin,sodium selenate,and were digested with trypsinase and collected as the single cell suspension.The cell viability was determined with trypan blue staining,the purity of olfactory ensheathing cells was identified with P75NGFR and S-100 double-labeled immunofluorescence staining.Results More typical olfactory ensheathing cells were found with multi-protruding shape or spindle-shape,less with bipolar or flat circular.The viability of olfactory ensheathing cells was more than 95%,the purity of the cells was more than 90%.Conclusion The cultured olfactory ensheathing cells might be available in clinical research.