Effects of Yinqiaosan on Influenza Virus Load and IFN-γ mRNA Expression in Mice

OBJECTIVE To investigate the effects of Yinqiaosan on influenza virus load and IFN-γ mRNA expression.METHODS The mice were divided into control group,influenza virus group,Yinqiaosan high dose group,Yinqiaosan middle dose group and Yinqiaosan low dose group.Real-time PCR technique was used to detect virus load and IFN-γ mRNA expression in mice lung at the 1st day,2nd day,3rd day,5th day and 7th day after being infected.RESULTS The high dose of Yinqiaosan decreased virus load at every time point(P0.05,P0.01).The middle and low dose decreased virus load at the 3rd day and 5th day(P0.01,P0.05).Lung IFN-γ mRNA expression was increased in high dose group at every time point(P0.01,P0.05),IFN-γ mRNA expression in middle dose group was increased at the 2nd day,3rd day and 5th day(P0.01),Yinqiaosan in low dose group can increase IFN-γ mRNA expression was increased in low dose group at 3rd and 5th day(P0.01).CONCLUSION Yinqiaosan exerts antiviral effects against influenza virus by upregulating expression of IFN-γ mRNA.