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Effects of insulin induced by hyperglycemia and the expression of bcl-x and bax in human umbilical vein endothelial cell

Ping Feng

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Abstract

Objective:To investigate the effects of insulin on human umbilical vein endothelial cells(HUVECs) induced by high glucose and analyze the expression changes of bcl-x and bax.Methods:HUVECs were isolated and incubated at different concentration of glucose and insulin for 72 hours,and total RNA was extracted.Using semi-quantitative RT-PCR techniques,we measured the expression of bcl-xL and bax mRNA in HUVECs.Results:High glucose could reduce the expression of bcl-xL and increase the expression of bax.After adding physiological or high concentration of insulin,the expressions of bcl-xL increased and the expressions of bax reduced.Conclusion:Insulin can increase HUVEC expression of bcl-xL and reduce the expression of bax.

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What this paper is about

Objective:To investigate the effects of insulin on human umbilical vein endothelial cells(HUVECs) induced by high glucose and analyze the expression changes of bcl-x and bax.Methods:HUVECs were isolated and incubated at different concentration of glucose and insulin for 72 hours,and total RNA was extracted.Using semi-quantitative RT-PCR techniques,we measured the expression of bcl-xL and bax mRNA in HUVECs.Results:High glucose could reduce the expression of bcl-xL and increase the expression of bax.After adding physiological or high concentration of insulin,the expressions of bcl-xL increased and the expressions of bax reduced.Conclusion:Insulin can increase HUVEC expression of bcl-xL and reduce the expression of bax.

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Available abstract

Objective:To investigate the effects of insulin on human umbilical vein endothelial cells(HUVECs) induced by high glucose and analyze the expression changes of bcl-x and bax.Methods:HUVECs were isolated and incubated at different concentration of glucose and insulin for 72 hours,and total RNA was extracted.Using semi-quantitative RT-PCR techniques,we measured the expression of bcl-xL and bax mRNA in HUVECs.Results:High glucose could reduce the expression of bcl-xL and increase the expression of bax.After adding physiological or high concentration of insulin,the expressions of bcl-xL increased and the expressions of bax reduced.Conclusion:Insulin can increase HUVEC expression of bcl-xL and reduce the expression of bax.

Key concepts: Umbilical vein, Insulin, Apoptosis, Messenger RNA, Bcl-xL, Human umbilical vein endothelial cell, Real-time polymerase chain reaction, Cell

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Effects of insulin induced by hyperglycemia and the expression of bcl-x and bax in human umbilical vein endothelial cell — Research Paper | ScholarLens