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[Sequence difference of ribosomal DNA second internal transcribed spacer in Anopheles minimus in different localities].

Shuisen Zhou, Lin-hua Tang, Zheng-cheng Gu, Yi Wang

Open publisher page 3 citations

Abstract

OBJECTIVE: To compare the sequence difference of the ribosomal DNA second internal transcribed spacer (ITS2) in Anopheles minimus from Yunnan, Hainan, Guangxi of China and from Thailand. METHODS: Single mosquito's legs were digested to extract DNA; the rDNA-ITS2 region was amplified by specific primers and the PCR products were purified, sequenced and analysed. RESULTS: Two different kinds of ITS2 sequences, temporarily named as An. minimus A and An. minimus C, were found in this study. The length and GC contents of ITS2 region in species A and species C are 481 bp, 54.04% and 483 bp, 54.23%, respectively. Two species differed by 5.8% in the ITS2 sequences owing to 22 fixed nucleotide substitutions and indels. CONCLUSION: Two cryptic species, An. minimus A and An. minimus C, were found in the research localities.

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What this paper is about

OBJECTIVE: To compare the sequence difference of the ribosomal DNA second internal transcribed spacer (ITS2) in Anopheles minimus from Yunnan, Hainan, Guangxi of China and from Thailand. METHODS: Single mosquito's legs were digested to extract DNA; the rDNA-ITS2 region was amplified by specific primers and the PCR products were purified, sequenced and analysed. RESULTS: Two different kinds of ITS2 sequences, temporarily named as An. minimus A and An. minimus C, were found in this study. The length and GC contents of ITS2 region in species A and species C are 481 bp, 54.04% and 483 bp, 54.23%, respectively. Two species differed by 5.8% in the ITS2 sequences owing to 22 fixed nucleotide substitutions and indels. CONCLUSION: Two cryptic species, An. minimus A and An. minimus C, were found in the research localities.

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Available abstract

OBJECTIVE: To compare the sequence difference of the ribosomal DNA second internal transcribed spacer (ITS2) in Anopheles minimus from Yunnan, Hainan, Guangxi of China and from Thailand. METHODS: Single mosquito's legs were digested to extract DNA; the rDNA-ITS2 region was amplified by specific primers and the PCR products were purified, sequenced and analysed. RESULTS: Two different kinds of ITS2 sequences, temporarily named as An. minimus A and An. minimus C, were found in this study. The length and GC contents of ITS2 region in species A and species C are 481 bp, 54.04% and 483 bp, 54.23%, respectively. Two species differed by 5.8% in the ITS2 sequences owing to 22 fixed nucleotide substitutions and indels. CONCLUSION: Two cryptic species, An. minimus A and An. minimus C, were found in the research localities.

Key concepts: Internal transcribed spacer, Biology, Ribosomal DNA, Indel, Spacer DNA, Ribosomal RNA, Genetics, DNA

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[Sequence difference of ribosomal DNA second internal transcribed spacer in Anopheles minimus in different localities]. — Research Paper | ScholarLens