Effects of HMGB1 on Proliferation,Collagen Types I and III mRNA Expression in Cardiac Fibroblasts

To investigate the effects of HMGB1(High mobility group protein-B1) on the proliferation and synthesis of collagen in cultured rat cardiac fibroblasts and evaluate its potential role in the process of cardiac remodeling after myocardial infarction Neonatal Sprague-Dawley rat cardiac fibroblasts were isolated,cultured and divided into 4 groups.They were treated in vitro which 0(control group),0.01,0.1 and 1 mg/L HMGB1 respectively.After 6,12,18,24 and 48 hours of treatment of HMGB1,the proliferation was determined by MTT assay and the mRNA of collagen was analyzed by Real-time quantitative RT-PCR Itis.It is resulted:(1)In contrast to the control group,the proliferation level and collagen mRNA in HMGB1 treated groups were higher after 48 hours of treatment.(p0.05).(2)A significant higher level of proliferation and collagen mRNA expression in 0.1 mg/L group is observed,as compared to other groups(p0.05).It is conclused that the present data suggests that HMGB1 can increase the proliferation,collagen mRNA expression of neonatal Sprague-Dawley rat cardiac fibroblasts in a concentration-dependent manner to some extent and HMGB1 may play an important role in cardiac remodeling after myocardial infarction.