Analysis of Genotype and Antibiotic Resistance of ESBLs-producing Klebsiella Pneumoniae

Objective To understand the profile of genotype and antibiotic resistance of strains producing extended spectrum beta-lactamases (ESBLs). Methods 46 ESBLs-producing Klebsiella pneumoniae strains were determined by phennotyic confirmatory test. MICs of antibiotic to the isolates were determined by the agar dilution method. The presence of the TEM, SHV and CTX-M genes were determined by specific PCR assays. Results MIC test show these strains were 100% susceptible to imipenem , 84.4% to cefepime,71.9% to piperacillin/tazobactam, 70.3% to ciprofloxacin.Of all ESBLs, 81.3% were SHV, 65.6% were CTX-M and 18.8% were TEM . 53.1% of strains carried more than one type of ESBLs gene. Conclusions ESBLs-producing Klebsiella pneumoniae showed most susceptibility to imipenem. The most commom ESBLs in Klebsiella pneumoniae was SHV in our hospital, and the next was CTX-M.