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[Study on the distribution of Yersinia enterocolitica in Nantong, Jinagsu Province].

Ling Gu, Hua Wang, Fengcai Zhu, Zhiyang Shi, Xiaoyan Zeng, Zhao-ying Tan, Yuchun Xiao, Haiyan Qiu, Xin Wang, Dong Jin, Zhigang Cui, Wang Bing, Biao Kan, Xinsheng Wang, Huaiqi Jing, Jianguo Xu

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Abstract

OBJECTIVE: To study the distribution of Yersinia enterocolitica and its virulence factors in Nantong, Jiangsu. METHODS: Yersinia strains were isolated from livestock and poultry. Conventional PCR was used to detect the virulence factors of all strains and strain 0:8 was analyzed by pulsed-field gel electrophoresis(PFGE). RESULTS: The combined isolation rate of Yersinia enterocolitica from livestock and poultry was 31.06% and the gene distribution characters were: 39.57% of them were ail-, ystA- , ystB-, yadA- , virF-; 60.43% were ail- , ystA- , ystB + , yadA- , virF- respectively. The two reference strains from America and Denmark showed similar electrophoresis patterns but were significantly different with O:8 strains isolated from China while the serotypes of Yersinia enterocolitica O:3 and O:9 which were the main epidemic strains in China, were not found in this area. CONCLUSION: The pathogenic Yersinia enterocolitis O:3 and O:9 were not found in Nantong,Jiangsu province.

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OBJECTIVE: To study the distribution of Yersinia enterocolitica and its virulence factors in Nantong, Jiangsu. METHODS: Yersinia strains were isolated from livestock and poultry. Conventional PCR was used to detect the virulence factors of all strains and strain 0:8 was analyzed by pulsed-field gel electrophoresis(PFGE). RESULTS: The combined isolation rate of Yersinia enterocolitica from livestock and poultry was 31.06% and the gene distribution characters were: 39.57% of them were ail-, ystA- , ystB-, yadA- , virF-; 60.43% were ail- , ystA- , ystB + , yadA- , virF- respectively. The two reference strains from America and Denmark showed similar electrophoresis patterns but were significantly different with O:8 strains isolated from China while the serotypes of Yersinia enterocolitica O:3 and O:9 which were the main epidemic strains in China, were not found in this area. CONCLUSION: The pathogenic Yersinia enterocolitis O:3 and O:9 were not found in Nantong,Jiangsu province.

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Available abstract

OBJECTIVE: To study the distribution of Yersinia enterocolitica and its virulence factors in Nantong, Jiangsu. METHODS: Yersinia strains were isolated from livestock and poultry. Conventional PCR was used to detect the virulence factors of all strains and strain 0:8 was analyzed by pulsed-field gel electrophoresis(PFGE). RESULTS: The combined isolation rate of Yersinia enterocolitica from livestock and poultry was 31.06% and the gene distribution characters were: 39.57% of them were ail-, ystA- , ystB-, yadA- , virF-; 60.43% were ail- , ystA- , ystB + , yadA- , virF- respectively. The two reference strains from America and Denmark showed similar electrophoresis patterns but were significantly different with O:8 strains isolated from China while the serotypes of Yersinia enterocolitica O:3 and O:9 which were the main epidemic strains in China, were not found in this area. CONCLUSION: The pathogenic Yersinia enterocolitis O:3 and O:9 were not found in Nantong,Jiangsu province.

Key concepts: Yersinia enterocolitica, Yersinia, Virulence, Serotype, Pulsed-field gel electrophoresis, Microbiology, Yersinia Infections, Biology

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