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Determination of quercetin, kaempferol,isorhamnetin and flavonoids in Compound Ginkgo Granules by HPLC

Chunqin Mao

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Abstract

AIM: To establish a method for determining total flavonoids in Compound Ginkgo Granules so as to control the quality. METHODS: HPLC conditions: YWG-C_ 18 column, mobile phase: V(MeOH)∶V(1%HAC)=50∶50, flow rate: 1.0 mL/min, detection:λ=360 nm, column temperature: 25 ℃. RESULTS: The calibration curve of quercetin、 kaempferol and isorhamnetin was linear in range of 0.057 2 -0.286 0 μg, 0.074 0 -0.370 0 μg, 0.029 4 -0.147 0 μg, respectively. The average recovery was 98.39% , and RSD was 1.25 (n=6). CONCLUSION: This method is rapid, accurate and reproducible and could be used for quality control of Compound Ginkgo Granules.

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AIM: To establish a method for determining total flavonoids in Compound Ginkgo Granules so as to control the quality. METHODS: HPLC conditions: YWG-C_ 18 column, mobile phase: V(MeOH)∶V(1%HAC)=50∶50, flow rate: 1.0 mL/min, detection:λ=360 nm, column temperature: 25 ℃. RESULTS: The calibration curve of quercetin、 kaempferol and isorhamnetin was linear in range of 0.057 2 -0.286 0 μg, 0.074 0 -0.370 0 μg, 0.029 4 -0.147 0 μg, respectively. The average recovery was 98.39% , and RSD was 1.25 (n=6). CONCLUSION: This method is rapid, accurate and reproducible and could be used for quality control of Compound Ginkgo Granules.

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Available abstract

AIM: To establish a method for determining total flavonoids in Compound Ginkgo Granules so as to control the quality. METHODS: HPLC conditions: YWG-C_ 18 column, mobile phase: V(MeOH)∶V(1%HAC)=50∶50, flow rate: 1.0 mL/min, detection:λ=360 nm, column temperature: 25 ℃. RESULTS: The calibration curve of quercetin、 kaempferol and isorhamnetin was linear in range of 0.057 2 -0.286 0 μg, 0.074 0 -0.370 0 μg, 0.029 4 -0.147 0 μg, respectively. The average recovery was 98.39% , and RSD was 1.25 (n=6). CONCLUSION: This method is rapid, accurate and reproducible and could be used for quality control of Compound Ginkgo Granules.

Key concepts: Isorhamnetin, Kaempferol, Ginkgo, Chromatography, Chemistry, Quercetin, High-performance liquid chromatography, Calibration curve

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Determination of quercetin, kaempferol,isorhamnetin and flavonoids in Compound Ginkgo Granules by HPLC — Research Paper | ScholarLens