Impact of gene transfection on the expression of vein endothelial cell

Objective To transfect the CD74 gene to ECV304 cells for observing the impact of transfecting HUVEC on the expression of CD74.Methods The expression of some function-associated genes(HLA-A,HLA-DR and IFNGR)before and after transfected ECV304 by Real-time PCR was detected.Results The △Ct values of HLA-A before and after transfecting CD74 gene to ECV304 were 9.4881 and 12.1097,and 2-△△Ct was 6.1543 showing that the expression of HLA-A was significantly different.The values of IFNGR and HLA-DR were 4.9828,5.5228,the 2-△△Ct was 1.4539,while the HLA-DR and △Ct were 14.1098 and 14.7924,2-△△Ct was 1.3036 recpectively showing that there was no signficant differences in the expressions of IFNGR and HLA-DR before and after transfection.Conclusion Human umbilical vein endothelial cell ECV304 can express HLA-A,HLA-DR,IFNGR and CD74 mRNA.ECV304 can increase the expression of CD74 gene and membrane molecules and it can also increase the expression of HLA-A gene,but it can't increase the expression of HLA-DR and IFNGR.