Comparative evaluation of HbA1c results detected by two methods of different principles

Objective To assess the accuracy of the immunoturbidimetry assay for detecting glycated hemoglo- bin A1c(HbA1c)with the high-performance liquid chromatography(HPLC)as the reference method.Methods 200 clinical blood samples collected from different degrees of diabetic patients and healthy people were performed the HbA1cblinded parallel detection by the Livzon HbA1cimmunoturbidimetry reagent and the BIO-RAD HPLC rea- gent.Results The highest and the lowest values of HbA1cin the results of this batch of samples were 13.7% and 4.5%respectively,there was no outlier needing to be excluded.We calculated several parameters by statistical analy- sis,including the linearity correlation coefficient(r=0.997 0),the intraclass correlation coefficient(ICC=0.996 6) and the fitting linear equation was(Y=0.999 8 X-0.017 8,r2=0.993 2).Conclusion The immunoturbidimetry as- say and HPLC have a good correlation and consistence for detecting HbA1c,and the former assay method is more suitable for promotion and application in primary hospitals because of its relative low reagent cost and no need of spe- cial instrument.