2013•Journal of clinical and experimental medicineRequires access
Study on effect of total flavonone of hawthorn leaf in treatment of memory impairment and inflammatory reaction in rats with chronic cerebral ischemia
Baohua Li
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Abstract
Objective To explore the efficacy of total flavanone of hawthorn leaf in treatment of memory impairment and inflammatory reaction in rats with chronic cerebral ischemia.Methods Sixty four SD male rats were randomly divided into sham operation group,model group,total flavanone of hawthorn leaf group and nimodipine group.Each group had 16 rats.The model of chronic cerebral ischemia was established by permanent ligation of bilateral carotid arteries.Total amount of flavanone of hawthorn leaf at dosage of 140mg / kg.d per rat was intragastrically administered for 36 days.The ability of learning and memory in these rats was evaluated by the eight-arm maze method.The content of TNF α and IL-1 in brain tissue was measured by radioimmunoassay.The expression of GFAP in brain tissue was detected by immunohistochemical method.Results The application of total flavanone of hawthorn leaf can improve the impairment of learning and memory in rats with chronic cerebral ischemia and reduce the number of errors in learning memory and working memory of rats(P 0.05),inhibit GFAP expression in brain tissue(P 0.05),and reduce the content of TNF-α and IL-1 in brain tissue(P 0.05).Conclusion Total flavanone of hawthorn leaf can reduce the content of TNF-α and IL-1 and decrease the inflammatory response of brain tissue by inhibition of GFAP expression in rats with chronic cerebral ischemia,thus,it may improve the capability of learning and memory in rats with chronic cerebral ischemia.