The effects of Nimesulide on cell proliferation and apoptosis inducing in human carcinoma of the colon cells

ObjectiveTo characterize the effects and it s mechanisms of Nimesulide in inducing growth inhibition and apoptosis in human carcinoma of the colon cell lines. MethodsUsing MTT method,Flow Cytometry (FCM),electron microsco py and immunohistochemisty,the effects of Nimesulide on the Eca-109 of prolifer ation and its mechanism were assayed.ResultsThe data showed that the growth of the HT-29 was inhibit ed by Nimesulide.The inhibitory rates for growth of the HT-29 were positively c orrelated with the concentration and duration of Nimesulide in the media.Sub-G 1 peak was detected by FCM,the ration of apoptosis was 6.28%-39.34%.The cell p ercent of S and G 2/M were increased,and that of G 0/G 1 phase decreased afte r treatment in dose-dependent manner.The HT-29 exhibited some morphologic feat ures of apoptosis,including cell shrinkage,nuclear condensation,DNA fragmentatio n and formation of apoptotic bodies by electron microscopy.After incubation with Nimesulide,the expression of COX-2 and Bcl-2 were reduced in colon carcinoma cells.ConclusionNimesulide inhibited the growth of human carcinoma of the esophagus cell line Eca-109 in vitro.The effect of the Nimesulide on phases of cell cycle and induceapoptosis,which was associated with regression of COC- 2 and Bcl-2 may be partially explained the cytotocix effects of Nimesulide.