Fermentative production of 1,3-propanediol from glycerol by Clostridium butyricum

The production of 1,3-propanediol from glycerol by Clostridium butyricum was studied and the anaerobic condition was strictly maintained in the fermentation. The medium were as follows: 6% glycerol, 1% glucose,2% corn steep liquor, 0.2%(NH 4) 2SO 4. The optimal pH for the fermentation medium was found to be between 6.5 and 7.0, and the optimal temperature was 34℃. Under the optimal conditions, 40.7g/L of 1,3-propanediol was obtained from 6% glycerol, with the molar yield of 68%.