2005•Unpublished venueRequires access
The Expression of Human Norpeg Protein in E.coli and Preparation of Ployclonal Antibody Against the Protein
Sun Yu-ning
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Abstract
Objective To express prokaryotically the domain C of Norpeg protein in E.coli, and prepare mouse polyclonal antibody against Norpeg. Methods The domain C of Norpeg gene was cloned into an prokaryotic expression vector pGEX4T-1, and then the recombinant vector was transformed into E.coli. The recombinant fusion protein was expressed in DH5α under IPTG induction. Mouse polyclonal antibody against (GST-Norpeg) fusion protein was prepared with purified GST-Norpeg fusion protein as immunogen. Results The prokaryotic expression vector was successfully constructed and purified from E.coli. The recombinant protein was expressed and polyclonal antibody against Norpeg was prepared. Conclusion Norpeg gene fragment can be expressed in E.coli, polyclonal antibody against Norpeg has higher specificity. It provides a powerful tool for further function study of Norpeg.