Effect of Soil Particle on Petroleum Microorganism Biodegradation Process

[Objective] The paper aimed to investigate the interactions occurring between soil particle and organic chemicals in petroleum bioremediation in soil.[Method] Bioremediation of particle soil(63 μm;63-2 000 μm) from different source was tested.[Result] TPH of new polluted soil was removed by 52.2%,and the TPH of deep soil was not removed effectively.Alkane were exhausted in all tests.The biodegradation on surface soil was obviously with soil aging.GC-FID analysis indicated TPH with ECN15-20 was difficult to be degraded,which was more obvious in deep oil than surface soil,indicating that the age of soil influenced the distribution of organic chemicals.In addition,the aged soil had the identical composition high molecular structure ECN15.[Conclusion] The study provided reference for the application of petroleum microorganism in bioremediation of soil polluted by petroleum.