ScholarLens Back to search
2013•Journal of Gannan Medical UniversityRequires access

The Effects of Resveratrol on Neuronal Apoptosis Induced by Hypoxia

Wei Chu-rong

Open publisher page 0 citations

Abstract

Objective:To explore the effects of resveratrol(RES) on neuronal apoptosis induced Hypoxia in cortical neurons.Methods: The present study established an hypoxic model in primary cultured cortical neurons of mice.In the hypoxia model,the level of ROS was determined using DCFH-DA and fluorescence microscopic imaging systems and apoptosis was analyzed by staining of propidium iodide(PI) and Hoechst 33342.Results: After pretreated with 25 μM RES for 60 min before hypoxia,the level of ROS and neuronal apoptosis were significantly lower than those in hypoxia cells.And pretreatment with 1 mM NAC(a ROS scavenger) can also reduce neuronal apoptosis percentage induced by hypoxia in neurons.Conclusion: The results indicated that RES could inhibit the hypoxia induced apoptosis in cortical neurons via reducing the level of ROS.

About this research paper

What this paper is about

Objective:To explore the effects of resveratrol(RES) on neuronal apoptosis induced Hypoxia in cortical neurons.Methods: The present study established an hypoxic model in primary cultured cortical neurons of mice.In the hypoxia model,the level of ROS was determined using DCFH-DA and fluorescence microscopic imaging systems and apoptosis was analyzed by staining of propidium iodide(PI) and Hoechst 33342.Results: After pretreated with 25 μM RES for 60 min before hypoxia,the level of ROS and neuronal apoptosis were significantly lower than those in hypoxia cells.And pretreatment with 1 mM NAC(a ROS scavenger) can also reduce neuronal apoptosis percentage induced by hypoxia in neurons.Conclusion: The results indicated that RES could inhibit the hypoxia induced apoptosis in cortical neurons via reducing the level of ROS.

Why it matters

A significance statement is not available in the OpenAlex record.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

Objective:To explore the effects of resveratrol(RES) on neuronal apoptosis induced Hypoxia in cortical neurons.Methods: The present study established an hypoxic model in primary cultured cortical neurons of mice.In the hypoxia model,the level of ROS was determined using DCFH-DA and fluorescence microscopic imaging systems and apoptosis was analyzed by staining of propidium iodide(PI) and Hoechst 33342.Results: After pretreated with 25 μM RES for 60 min before hypoxia,the level of ROS and neuronal apoptosis were significantly lower than those in hypoxia cells.And pretreatment with 1 mM NAC(a ROS scavenger) can also reduce neuronal apoptosis percentage induced by hypoxia in neurons.Conclusion: The results indicated that RES could inhibit the hypoxia induced apoptosis in cortical neurons via reducing the level of ROS.

Key concepts: Propidium iodide, Apoptosis, Hypoxia (environmental), Resveratrol, Medicine, Fluorescent staining, Reactive oxygen species, Staining

Related papers

Related paper 1Related paper 2Related paper 3
Back to paper searchBrowse research topicsOriginal source
The Effects of Resveratrol on Neuronal Apoptosis Induced by Hypoxia — Research Paper | ScholarLens