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Determination of quercetin in microwave assisted extraction of Flos Sophorae immaturus by HPLC

Chen Dai-wu

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Abstract

OBJECTIVE To optimize the extracting procedure for quercetin from Flos Sophorae immaturus.METHODS The yield of quercetin of microwave assisted extraction (MAE),heating reflux extraction and soaking extraction from Flos Sophorae immaturu were studied. HPLC was used to determine the content of quercetin in extracts. And the orthogonal experimental design was used to optimize the conditions of MAE.RESULTS The optimized conditions of MAE were as follows: the sample was 60-90 meshed,with 25% moisture and the power of microwave irradiation was 350 W,with extraction time of 20 min.CONCLUSION MAE is a rapid and efficient extraction technique for Flos Sophorae immaturus.

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What this paper is about

OBJECTIVE To optimize the extracting procedure for quercetin from Flos Sophorae immaturus.METHODS The yield of quercetin of microwave assisted extraction (MAE),heating reflux extraction and soaking extraction from Flos Sophorae immaturu were studied. HPLC was used to determine the content of quercetin in extracts. And the orthogonal experimental design was used to optimize the conditions of MAE.RESULTS The optimized conditions of MAE were as follows: the sample was 60-90 meshed,with 25% moisture and the power of microwave irradiation was 350 W,with extraction time of 20 min.CONCLUSION MAE is a rapid and efficient extraction technique for Flos Sophorae immaturus.

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Available abstract

OBJECTIVE To optimize the extracting procedure for quercetin from Flos Sophorae immaturus.METHODS The yield of quercetin of microwave assisted extraction (MAE),heating reflux extraction and soaking extraction from Flos Sophorae immaturu were studied. HPLC was used to determine the content of quercetin in extracts. And the orthogonal experimental design was used to optimize the conditions of MAE.RESULTS The optimized conditions of MAE were as follows: the sample was 60-90 meshed,with 25% moisture and the power of microwave irradiation was 350 W,with extraction time of 20 min.CONCLUSION MAE is a rapid and efficient extraction technique for Flos Sophorae immaturus.

Key concepts: Flos, Chemistry, Quercetin, Extraction (chemistry), Chromatography, High-performance liquid chromatography, Rutin, Yield (engineering)

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