A new method for PCR products cloning - T-A clone technique

A new method for PCR products cloning T A cloning technique is introduced. pBluescript SK(+) plasmid was extracted by modified alkali lysis method. A blunt ends was generated using restriction enzyme Eco RⅤ, then T A cloning vector was prepared in the presence of Taq DNA polymerase and dTTP. β actin cDNA fragment was cloned into the above T A cloning vector. After the recombinant plasmids were digested by Eco RⅠ and Hin dⅢ, an expected size of β actin cDNA fragment was obtained .