CTNNB1 gene silence effect inhibit the growth of human colon cancer SW480 cells

Objective To observe the inhibitory effect of CTNNB1 gene expression and cell proliferation of the human colon cancer cell line SW480.Methods The shRNA plasmid vector against CTNNB1 was constructed and transfected into SW480 cells with LipofectamineTM2000.The down-regulations of CTNNB1 expressions were detected by RT-PCR and western blot analysis.The cell proliferation inhibitions were determined by MTT assay.Results The shRNA vectors targeted against CTNNB1 were successfully constructed and efficiently suppressed the expression of CTNNB1 mRNA and protein(P0.05).The expression inhibition rates were 38.29% and 42.86% at the mRNA and protein level respectively.The MTT assay indicated that the specific shRNA resulted in significant inhibition of cell growth on the culture plates on a time-dependent manner.At 72 h post-transfection,the cell viability of CTN group was 43.7%,which was of statistical significance when compared with that of blank control group(P0.05).Conclusion The CTNNB1 gene silence effect could down-regulate the expression of CTNNB1 gene and inhibit the growth of SW480 cells.