ScholarLens Back to search
2008•Huaxi yaoxue zazhiRequires access

Determination of rutin and quercetin in A.hendersonii Hook f. by HPLC

Hong Xia Zheng

Open publisher page 0 citations

Abstract

OBJECTIVE To develop a method for determining rutin and quercetin in A.hendersonii Hook f.METHODS HPLC method was adopted.Agilent Zorbax SB-C18(250 mm ×4.6 mm,5 μm)column and methanol–0.04% phosphoric acid (40:60) as mobile phase were used.The flow rate was 1.0 ml·min-1 and the detection wavelength was 360 nm.The column temperature was 35℃.RESULTS The peak areas vs the concentrations of rutin and quercetin showed good correlation in the ranges of 0.01–0.20 μg and 0.007–0.099 μg(r=0.9999 and 0.9998),respectively.The average recoveries was 102.1% for quercetin (RSD=2.0%,n=6) and 99.5% for rutin (RSD=1.4%,n=6),respectively.CONCLUSION The method is simple,rapid and accurate.It can be used for the quality control of A.hendersonii Hook f..

About this research paper

What this paper is about

OBJECTIVE To develop a method for determining rutin and quercetin in A.hendersonii Hook f.METHODS HPLC method was adopted.Agilent Zorbax SB-C18(250 mm ×4.6 mm,5 μm)column and methanol–0.04% phosphoric acid (40:60) as mobile phase were used.The flow rate was 1.0 ml·min-1 and the detection wavelength was 360 nm.The column temperature was 35℃.RESULTS The peak areas vs the concentrations of rutin and quercetin showed good correlation in the ranges of 0.01–0.20 μg and 0.007–0.099 μg(r=0.9999 and 0.9998),respectively.The average recoveries was 102.1% for quercetin (RSD=2.0%,n=6) and 99.5% for rutin (RSD=1.4%,n=6),respectively.CONCLUSION The method is simple,rapid and accurate.It can be used for the quality control of A.hendersonii Hook f..

Why it matters

A significance statement is not available in the OpenAlex record.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

OBJECTIVE To develop a method for determining rutin and quercetin in A.hendersonii Hook f.METHODS HPLC method was adopted.Agilent Zorbax SB-C18(250 mm ×4.6 mm,5 μm)column and methanol–0.04% phosphoric acid (40:60) as mobile phase were used.The flow rate was 1.0 ml·min-1 and the detection wavelength was 360 nm.The column temperature was 35℃.RESULTS The peak areas vs the concentrations of rutin and quercetin showed good correlation in the ranges of 0.01–0.20 μg and 0.007–0.099 μg(r=0.9999 and 0.9998),respectively.The average recoveries was 102.1% for quercetin (RSD=2.0%,n=6) and 99.5% for rutin (RSD=1.4%,n=6),respectively.CONCLUSION The method is simple,rapid and accurate.It can be used for the quality control of A.hendersonii Hook f..

Key concepts: Rutin, Chemistry, Quercetin, Phosphoric acid, Chromatography, High-performance liquid chromatography, Methanol, Biochemistry

Related papers

Related paper 1Related paper 2Related paper 3
Back to paper searchBrowse research topicsOriginal source
Determination of rutin and quercetin in A.hendersonii Hook f. by HPLC — Research Paper | ScholarLens