Application of ATP-bioluminescence Assay for Screening Chemotherapeutic Agents of Ovarian Cancer Chemotherapy

To evaluate the feasibility of using ATP-bioluminescence assay for tumor chemosensitivity testing in vitro, we selected the A2780 cell line as a model and established the suitable assay condition. Screening chemotherapeutic agents of ovarian cancer in vitro were preliminarily researched. Using this assay, dose-response curve was detected in cell line treated with these agents. The result showed that the coefficients of variation for assay ranged from 0.2% to 8.2%, which means high reproducibility.It can measured ATP content of as few as forty cells. The thermal stability of the luciferin-luciferase system was high enough used in industry. The predictable accuracy rate is about 90.6%. This study demonstrated ATP-bioluminescence assay is a reliable, reproducible and sensitive method.It can provide a technical base for screening sensitive chemotherapeutic agents in clinic.