Exogenous Id3 gene inhibits the biological activity of human lung adenocarcinoma A549 cells

Objective The inhibitor of differentiation 3( Id3),as an important transcription regular factor,participates in such processes as tumorigenesis,cell proliferation and cell apoptosis. This article aimed to evaluate the effects of Id3 on the migration,invasion and proliferation of A549 cells. Methods Recombinant eukaryotic expression vector pEGFP / Id3 was constructed and transfected into A549 cells by the liposome-mediated method. The expression of pEGFP / Id3 in A549 cells was analyzed by fluorescence microscopy and RT-PCR. The effects of Id3 on the invasion and migration of A549 cells were detected using the scratch test and invasion chamber model,and that on the proliferation of A549 cells was observed by plate colony formation assay. Results The eukaryotic expression vector pEGFP / Id3 was successfully transfected into the A549 cells. Id3 / GAPDH in pEGFP / Id3 was transfection group 1. 274 ± 0. 035, which was higher than that in pEGFP transfection group( 0. 584 ± 0. 011) and control group( 0. 636 ± 0. 035). Id3 was effectively expressed in the A549 cells and significantly reduced their migration and invasion abilities. The transference number of the cells in pEGFP / Id3 group at 48 hours( [113. 75 ± 25. 04]) was lower than that in control group( [254. 75 ± 41. 06]) and pEGFP transfection group( [228.25 ±26.84])( P 0.05). Plate colony formation assay showed the exogenous Id3 gene transfection evidently inhibited the proliferation of the A549 cells. The success rate of pEGFP/ Id3 transfection group( [15. 67 ± 5. 75]%) was lower significantly than that in control group( [37. 83 ± 4. 16]%)( P 0. 05). Conclusion The exogenous Id3 gene can inhibit the metastasis and proliferation of A549 cells.