Inhibitory effect of oxymatrine on high glucose-induced rat renal tubular epithelial-mesenchymal transition

AIM: To investigate the inhibitory effect of oxymatrine( OM) on high glucose-induced rat renal tubular epithelial-mesenchymal transition( EMT). METHODS: The rat renal tubular epithelial NRK52E cells were cultured in vitro. The cells were divided into control group,high glucose group,high glucose + different concentrations of OM groups and high glucose + 0. 50 g / L OM dynamic observation group. The expression of TGF-β 1,Smad7,α-SMA and E-cadherin at mRNA and protein levels was detected by real-time PCR and Western blotting. The viability of NRK52E cells was determined by MTT assay. RESULTS:( 1) Compared with control group,the expression of TGF-β 1 and α-SMA at mRNA and protein levels in high glucose group gradually increased,and Smad7 protein and E-cadherin mRNA and protein gradually reduced,but the mRNA expression of Smad7 gradually increased.( 2) Compared with high glucose group,as increases in OM doses,the expression of TGF-β 1 and α-SMA at mRNA and protein levels in high glucose + different concentrations of OM groups gradually reduced,and Smad7 protein and E-cadherin mRNA and protein gradually increased,but the mRNA expression of Smad7 had no significant change.( 3) Compared with high glucose group,the expression of TGF-β 1 and αSMA at mRNA and protein levels was significantly reduced,the expression of E-cadherin at mRNA and protein levels significantly increased,and the protein expression of Smad7 significantly increased,but the mRNA expression of Smad7 had no significant change in high glucose + 0. 50 g / L OM dynamic observation group. CONCLUSION: In NRK52E cells, oxymatrine inhibits high glucose induced EMT by down-regulating TGF-β 1 and up-regulating Smad7,thus preventing the fibrosis effect of TGF-β 1 / Smads signaling.