Cloning and expression of the extracellular domain of human CD38 antigen gene

Aim To clone and express the 799 bp cDNA fragment of the extracellular domain of human CD38. Methods The cDNA of the human CD38 antigen was amplified from total RNA of Daudi cells by RT PCR, and it was inserted into pGEM T. The cDNA fragment of human CD38 was amplified from the recombinant vector pGEM T, then it was subcloned into expression vector pET28a(+). E. coli BL21 was transfected with recombinant vector and expression was induced with IPTG. Results DNA sequencing showed that cloned 799 bp cDNA sequence was identical with having reported it. The target gene was expressed in E. coli . Conclusion The expressed product of 799 bp cDNA fragment of human CD38 obtained lay the foundation for studing the function of human CD38 antigen and producing anti CD38 monoclonal antibody.