CLONING AND SEQUENCING OF α-AMYLASE GENE FROM TWO WILD BACILLUS SUBTILIS STRAINS

0Two α-amylase-producing wild-type strains of Bacillus subtilis QSH4 and Bacillus subtilis SHX4 were screened from soil.The α-amylase genes from these strains were amplified via PCR technique using primers designed according to nucleotide sequence of α-amylase gene of Bacillus subtilis 168 strain.Sequence result showed that the genes from both strains were composed of 1948bp,and had an identity of 99.90%.They were shorter than α-amylase gene of 168 strain by 32bp(near the 3′ terminal) and had an identity of 96.97% with it.The predicted proteins for α-amylase genes of QSH4 and SHX4 strain were 477 amino acids long and 183 amino acids shorter than the α-amylase protein of 168 strain.As an inference from the result that the active site of the enzyme would be in three quarters of the N-terminal peptide.