Two-step hydrolysis of wheat germ protein with double-enzyme and antioxidant activity of hydrolysate

The previous study showed that neutral protease was the optimal single enzyme to hydrolyze wheat germ protein to prepare antioxidant peptides.On this basis,the experiment was conducted to study the process parameters on enzymatic hydrolysis and antioxidant activity of hydrolysates by double-enzyme.The results showed that the neutral protease and the alkaline protease were optimum combination.After hydrolysising 270 min by neutral protease,the enzymatic hydrolysate was acted upon by alkaline protease.The reducing power and the peptide concentration were increased differently when hydrolysis 300 min and 420 min.However,the scavenging rate of hydrolysates against DPPH free radicals showed an down trend throughout.Therefore,contrary to the single enzyme to hydrolyze wheat germ protein,double-enzyme had no vantage.