Simultaneous Determination of Five Anti-epileptic Drugs in Human Plasma by HPLC

Objective To establish a method for simultaneous determination of 5 anti-epileptic drugs in human plasma by high performance liquid chromatography( HPLC). Methods The analysis was performed with an Agilent Eclipse XDB-C18analytical column( 4. 6 mm × 150 mm,5μm) by gradient elution of water and methanol. The flow rate was 1 mL·min- 1. The detection wavelengths were set at 210 nm during 0 to 10 min and 230 nm during 10. 01 to 15 min. Results The linear ranges of diazepam,clonazepam,carbamazepine,phenytoin sodium and phenobarbital were 0. 1- 20. 0μg · mL- 1,0. 01-10. 0 μg·mL- 1,0. 2-40μg·mL- 1,2-40μg·mL- 1and 2- 80 μg·mL- 1,respectively. All the calibration curves showed good linearity( r≥0. 997 4) in the concentration ranges. The RSD of intra-and inter-day of the 5 analytes was less than 15%,and the recoveries were 74. 4%- 91. 3%. Conclusion The developed HPLC assay is accurate,sensitive and reliable,and it can be readily utilized as a reference method for rapid clinical determination.