Induction of the Proliferative Responses in T Lymphocytes of Patients with Multiple Sclerosis Induced by Different Doses of Myelin or Its Compositions

To explore the dose response relation of the proliferative responses in T lymphocytes of patients with multiple sclerosis induced with different doses of myelin or its compositions,the proliferative responses of human T cells from mononuclear cells of HLA DR15 type were induced in vitro with 6 peptide antigens including diluted concentrations of intact myelin,delipidated myelin,myelin basic protein (MBP),proteolipid protein (PLP),PLP178 191 and MBP87 106 Experimental results showed that the optimal concentrations for induction of responses induced by myelin and delipidated myelin were 50～150 μg/ml;those induced by PLP or MBP were 25～100 μg/ml,and those induced by various peptides were 20～50 μg/ml The proliferative response induced by myelin protein was stronger than those induced by myelin peptides,and the maximal response was observed in case induced by delipidated myelin with significant difference between T cells of patients with multiple sclerosis and the controls. It concludes that the proliferative responses exist in T lymphocytes of patients with multiple sclerosis induced by myelin antigens