Simultaneous Determination of 20 Anti-Obesity Drugs Illegally Added in Weight-loss Functional Foods by Matrix Solid Phase DispersionHigh Performance Liquid Chromatography Tandem Mass Spectrometry

An analytical method based on high performance liquid chromatography tandem mass spectrometry has been developed for the simultaneous determination of 20 anti-obesity drugs( fenfluramine,phenylpropanolamine,sibutramine,sertraline,rimonabant,bupropion,citalopram,fluoxetine,benfluorex,topiramate, zonisamide, caffeine, phenolphthalein, emodin, indapamide, bumetanide, torasemide,triamterene,orlistat,phenformin). that were extracted from various weight-loss functional foods by ethanolacetone( 7∶ 3,V /V) and purified by primary secondary amine( PSA) and octadecyltrimethoxysilane( ODS)under ultrasonication. The analysis was carried out on HPLC-MS /MS by electrospray ionization using multiple reaction monitoring after the chromatographic separation on Waters Atlantis T3( 3 μm,150 mm × 2. 1 mm)column. Identification was achieved by the retention time and the ion ratio,quantification was done by the external standard method. The limits of detection for the appetite suppressants were 0. 05-3. 0 mg /kg. The mean recoveries at the three spiked levels were 67. 1%-101. 4%,with the intra-day precision less than 10%and the inter-day precision less than 15%. The method is reliable,accurate,reproducible and suitable for the determination of the anti-obesity drugs in different weight-loss functional foods.