2010•Zhonghua zhongliu fangzhi zazhiRequires access
Effects of E-selectin and its ligand SLeX in mediating early adhesion of colorectal carcinoma
Sun Qing
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Abstract
OBJECTIVE:To study the role of E-selectin and SLeX in the adhesion between colorectal carcinoma LoVo cells and endothelial cell ECV304.METHODS:Using the immunocytochemisty method of strepto-avidin-biotin peroxidase complex (SP) detected the expression of E-selectin in human endothelial ECV304 cells;The adhesion of LoVo cells to human umbilical vein endothelial cells (ECV304) was detected by tumor cells uptaking Rose Bengal.RESULTS:The expression of E-selectin in Human umbilical vein endothelial cells (ECV304) and SLeX in LoVo cells were observed;After ECV304 cells were stimμLated by TNF-α,the adhesion of LoVo cells to ECV304 was more increased (P0.001),and the effect was time and TNF-αdensity dependent;After LoVo cells and ECV304 cells were exposed to different anti-E-selectin and anti-SLeX mAbs concentrences respectively,the cell relative adhesion rate decreased with anti-E-selectin and anti-SLeX mAbs concentrences(P0.001).The cell adhesion between LoVo and ECV304 coμLd be blocked by anti-E-selectin and anti-SLeX mAbs(P0.05).CONCLUSION:E-selectin and its ligand SLeX play an important role in mediating the adhesion reaction between LoVo cells and vascμLar endothelial cells.