Study on enzymatic hydrolysis of pig bone protein by neutral proteinase and antioxidant activities of its hydrolysate

Four main factors,including enzyme concentration,pH,enzymolysis temperature and enzymolysis time were firstly analyzed by using single factor analysis method.Then the optimal hydrolysis condition was obtained by orthogonal experiment.At last,the scavenging effect of the enzymatic hydrolysate on OH free radical were studied.The results showed that the optimum extraction conditions were obtained as follows:enzyme concentration 4%,pH 6.5,enzymolysis temperature 45℃ and enzymolysis time 3h.The degree of hydrolysis was 31.3%.When the concentrations were 133~4000μg/mL,the clearance rates of·OH free radical were 29.59%~81.43%,and the scavenging activity was correlated with the concentrations.But,the scavenging activity of enzymatic hydrolysis product was not as good as VC.